睾丸生殖细胞肿瘤病理亚型的综合特征：基因表达、突变和可变剪接（表1）

数据集获取（Figshare）

• 源站标题：Table_1_Comprehensive characteristics of pathological subtypes in testicular germ cell tumor: Gene expression, mutation and alternative splicing.docx
• DOI：10.3389/fimmu.2022.1096494.s004
• 发布日期：2023-01-13
• 文件数：1 个
• 文件总体积：19.83 KB
• 源站页面：https://frontiersin.figshare.com/articles/dataset/Table_1_Comprehensive_characteristics_of_pathological_subtypes_in_testicular_germ_cell_tumor_Gene_expression_mutation_and_alternative_splicing_docx/21895530

命令行下载

curl -L -o "Table_1_Comprehensive characteristics of pathological subtypes in testicular germ cell tumor: Gene expression, mutation and alternative splicing.docx" "https://ndownloader.figshare.com/files/38836113"

文件清单

• Table_1_Comprehensive characteristics of pathological subtypes in testicular germ cell tumor: Gene expression, mutation and alternative splicing.docx（19.83 KB）

文件总体积 19.83 KB（源站 API 实测）。

数据加载示例（图像类）

from PIL import Image
import glob, os

files = (glob.glob(os.path.join(path, "**", "*.png"), recursive=True)
       + glob.glob(os.path.join(path, "**", "*.jpg"), recursive=True)
       + glob.glob(os.path.join(path, "**", "*.tif"), recursive=True))
print("图像文件数:", len(files))
img = Image.open(files[0]); print("尺寸/模式:", img.size, img.mode)

# torchvision Dataset 方式:
# from torchvision import datasets
# ds = datasets.ImageFolder(path)  # 要求 子目录=类别

目录组织与标注格式以源站说明和下载后实际文件为准。

数据说明

BackgroundTesticular germ cell tumor (TGCT) is the most common tumor in young men, but molecular signatures, especially the alternative splicing (AS) between its subtypes have not yet been explored.
MethodsTo investigate the differences between TGCT subtypes, we comprehensively analyzed the data of gene expression, alternative splicing (AS), and somatic mutation in TGCT patients from the TCGA database. The gene ontology (GO) enrichment analyses were used to explore the function of differentially expressed genes and spliced genes respectively, and Spearman correlation analysis was performed to explore the correlation between differential genes and AS events. In addition, the possible patterns in which AS regulates gene expression were elaborated by the ensemble database transcript atlas. And, we identified important transcription factors that regulate gene expression and AS and functionally validated them in TGCT cell lines.
ResultsWe found significant differences between expression and AS in embryonal carcinoma and seminoma, while mixed cell tumors were in between. GO enrichment analyses revealed that both differentially expressed and spliced genes were enriched in transcriptional regulatory pathways, and obvious correlation between expression and AS events was determined. By analyzing the transcript map and the sites where splicing occurs, we have demonstrated that AS regulates gene expression in a variety of ways. We further identified two pivot AS-related molecules (SOX2 and HDAC9) involved in AS regulation, which were validated in embryonal carcinoma and seminoma cell lines. Differences in somatic mutations between subtypes are also of concern, with our results suggesting that mutations in some genes (B3GNT8, CAPN7, FAT4, GRK1, TACC2, and TRAM1L1) occur only in embryonal carcinoma, while mutations in KIT, KARS, and NRAS are observed only in seminoma.
ConclusionsIn conclusion, our analysis revealed the differences in gene expression, AS and somatic mutation among TGCT subtypes, providing a molecular basis for clinical diagnosis and precise therapy of TGCT patients.

许可

CC BY 4.0

引用

学术使用请引用 DOI 10.3389/fimmu.2022.1096494.s004（source: https://frontiersin.figshare.com/articles/dataset/Table_1_Comprehensive_characteristics_of_pathological_subtypes_in_testicular_germ_cell_tumor_Gene_expression_mutation_and_alternative_splicing_docx/21895530）。