下调lncRNA CCDC26通过上调IGF-1R导致人胃肠道间质瘤伊马替尼耐药

数据集获取（Figshare）

• 源站标题：Downregulation of lncRNA CCDC26 contributes to imatinib resistance in human gastrointestinal stromal tumors through IGF-1R upregulation
• DOI：10.6084/m9.figshare.8227448.v1
• 发布日期：2019-06-05
• 文件数：4 个
• 文件总体积：253.04 KB
• 源站页面：https://scielo.figshare.com/articles/dataset/Downregulation_of_lncRNA_CCDC26_contributes_to_imatinib_resistance_in_human_gastrointestinal_stromal_tumors_through_IGF-1R_upregulation/8227448

命令行下载

curl -L -o "1414-431X-bjmbr-52-6-e8399-gf001.jpg" "https://ndownloader.figshare.com/files/15336095"
curl -L -o "1414-431X-bjmbr-52-6-e8399-gf002.jpg" "https://ndownloader.figshare.com/files/15336104"
curl -L -o "1414-431X-bjmbr-52-6-e8399-gf003.jpg" "https://ndownloader.figshare.com/files/15336113"
curl -L -o "1414-431X-bjmbr-52-6-e8399-gf004.jpg" "https://ndownloader.figshare.com/files/15336122"

文件清单

• 1414-431X-bjmbr-52-6-e8399-gf001.jpg（54.96 KB）
• 1414-431X-bjmbr-52-6-e8399-gf002.jpg（70.60 KB）
• 1414-431X-bjmbr-52-6-e8399-gf003.jpg（91.35 KB）
• 1414-431X-bjmbr-52-6-e8399-gf004.jpg（36.13 KB）

文件总体积 253.04 KB（源站 API 实测）。

数据说明

Imatinib is the first line of therapy for patients with metastatic or gastrointestinal stromal tumors (GIST). However, drug resistance limits the long-term effect of imatinib. Long non-coding RNAs (lncRNAs) are emerging as key players in regulating drug resistance in cancer. In this study, we investigated the association between lncRNA CCDC26 and IGF-1R in GIST and their involvement in drug resistance. Considering the key role of lncRNAs in drug resistance in cancer, we hypothesized that IGF-1R is regulated by lncRNAs. The expression of a series of reported drug resistance-related lncRNAs, including CCDC26, ARF, H19, NBR2, NEAT1, and HOTAIR, in GIST cells treated with imatinib H19 was examined at various time-points by qRT-PCR. Based on our results and published literature, CCDC26, a strongly down-regulated lncRNA following imatinib treatment, was chosen as our research target. GIST cells with high expression of CCDC26 were sensitive to imatinib treatment while knockdown of CCDC26 significantly increased the resistance to imatinib. Furthermore, we found that CCDC26 interacted with c-KIT by RNA pull down, and that CCDC26 knockdown up-regulated the expression of IGF-1R. Moreover, IGF-1R inhibition reversed CCDC26 knockdown-mediated imatinib resistance in GIST. These results indicated that treatments targeting CCDC26-IGF-1R axis would be useful in increasing sensitivity to imatinib in GIST.

许可

CC BY 4.0

引用

学术使用请引用 DOI 10.6084/m9.figshare.8227448.v1（source: https://scielo.figshare.com/articles/dataset/Downregulation_of_lncRNA_CCDC26_contributes_to_imatinib_resistance_in_human_gastrointestinal_stromal_tumors_through_IGF-1R_upregulation/8227448）。