N-cadherin 动态调控复杂环境中儿童胶质瘤细胞迁移

数据集获取

源站：https://search.dataone.org/view/sha256%3A2df144f0871a48fcebdb7c7263ae738c0f42e7856a0ac8a5b5d80024ca206890

标准获取流程：

1. 访问源站链接，注册/登录账号；
2. 进入数据集详情页，按页面指引申请权限或直接下载；
3. 部分平台提供 SDK，例如：
   • OpenDataLab: pip install openxlab 后 openxlab.dataset.get(...)
   • ModelScope: pip install modelscope 后 modelscope download
   • 天池: 登录后页面提供直链下载
     以源站「数据下载/使用文档」页面为准。

数据说明

N-cadherin dynamically regulates pediatric glioma cell migration in complex environments Table S2. Control vs. N-cadherin shRNAs. We compared RNA transcriptomes from bulk cell populations, control or N-cadherin-depleted pediatric high-grade glioma cells. N-cad depletion decreased RNA expression but unaffected the expression of other cadherins and integrins except for CDH3. Differential gene expression analysis was performed with the DEseq2 for paired sample R package. Table S3. Leader vs. follower cells. We compared RNA transcriptomes from migrating glioma leader and follower cells that we isolated by photoconversion and flow cytometry. 44 gene transcripts increased and 36 decreased in leader relative to followers out of 19,729 genes that were quantified (log2 fold-change >0.5, FDR <0.05). YAP-response genes and wound-healing genes were higher in leader than follower cells. Differential gene expression analysis was performed with the DEseq2 for paired sample R package. NA r…, Pediatric high-grade gliomas are highly invasive and essentially incurable. Glioma cells migrate between neurons and glia, along axon tracts, and through extracellular matrix surrounding blood vessels and underlying the pia. Mechanisms that allow adaptation to such complex environments are poorly understood. N-cadherin is highly expressed in pediatric gliomas and is associated with shorter survival. We found that inter-cellular homotypic N-cadherin interactions differentially regulate glioma migration according to the microenvironment, stimulating migration on cultured neurons or astrocytes but inhibiting invasion into reconstituted or astrocyte-deposited extracellular matrix. N-cadherin localizes to filamentous connections between migrating leader cells but to epithelial-like junctions between followers. Leader cells have more surface and recycling N-cadherin, increased YAP1/TAZ signaling, and increased proliferation relative to followers. YAP1/TAZ signaling is dynamically regulated as…

数据加载示例（图像类）

from PIL import Image
import glob, os

files = (glob.glob(os.path.join(path, "**", "*.png"), recursive=True)
       + glob.glob(os.path.join(path, "**", "*.jpg"), recursive=True)
       + glob.glob(os.path.join(path, "**", "*.tif"), recursive=True))
print("图像文件数:", len(files))
img = Image.open(files[0]); print("尺寸/模式:", img.size, img.mode)

# torchvision Dataset 方式:
# from torchvision import datasets
# ds = datasets.ImageFolder(path)  # 要求 子目录=类别

目录组织与标注格式以源站说明和下载后实际文件为准。