荧光报告实验提供直接、准确、定量测量人类细胞中MGMT状态的数据集

数据集获取（Figshare）

• 源站标题：Fluorescent reporter assays provide direct, accurate, quantitative measurements of MGMT status in human cells
• DOI：10.1371/journal.pone.0208341
• 发布日期：2019-02-27
• 文件数：5 个
• 文件总体积：10.27 MB
• 源站页面：https://plos.figshare.com/articles/dataset/Fluorescent_reporter_assays_provide_direct_accurate_quantitative_measurements_of_MGMT_status_in_human_cells/7777952

命令行下载

curl -L -o "pone.0208341.s001.pdf" "https://ndownloader.figshare.com/files/14481380"
curl -L -o "pone.0208341.s002.tiff" "https://ndownloader.figshare.com/files/14481383"
curl -L -o "pone.0208341.s003.pdf" "https://ndownloader.figshare.com/files/14481386"
curl -L -o "pone.0208341.s004.docx" "https://ndownloader.figshare.com/files/14481389"
curl -L -o "pone.0208341.s005.docx" "https://ndownloader.figshare.com/files/14481392"

文件清单

• pone.0208341.s001.pdf（1.19 MB）
• pone.0208341.s002.tiff（9.00 MB）
• pone.0208341.s003.pdf（38.70 KB）
• pone.0208341.s004.docx（14.63 KB）
• pone.0208341.s005.docx（22.97 KB）

文件总体积 10.27 MB（源站 API 实测）。

数据加载示例（图像类）

from PIL import Image
import glob, os

files = (glob.glob(os.path.join(path, "**", "*.png"), recursive=True)
       + glob.glob(os.path.join(path, "**", "*.jpg"), recursive=True)
       + glob.glob(os.path.join(path, "**", "*.tif"), recursive=True))
print("图像文件数:", len(files))
img = Image.open(files[0]); print("尺寸/模式:", img.size, img.mode)

# torchvision Dataset 方式:
# from torchvision import datasets
# ds = datasets.ImageFolder(path)  # 要求 子目录=类别

目录组织与标注格式以源站说明和下载后实际文件为准。

数据说明

The DNA repair protein O6-methylguanine DNA methyltransferase (MGMT) strongly influences the effectiveness of cancer treatment with chemotherapeutic alkylating agents, and MGMT status in cancer cells could potentially contribute to tailored therapies for individual patients. However, the promoter methylation and immunohistochemical assays presently used for measuring MGMT in clinical samples are indirect, cumbersome and sometimes do not accurately report MGMT activity. Here we directly compare the accuracy of 6 analytical methods, including two fluorescent reporter assays, against the in vitro MGMT activity assay that is considered the gold standard for measuring MGMT DNA repair capacity. We discuss the relative advantages of each method. Our data indicate that two recently developed fluorescence-based assays measure MGMT activity accurately and efficiently, and could provide a functional dimension to clinical efforts to identify patients who are likely to benefit from alkylating chemotherapy.

许可

CC BY 4.0

引用

学术使用请引用 DOI 10.1371/journal.pone.0208341（source: https://plos.figshare.com/articles/dataset/Fluorescent_reporter_assays_provide_direct_accurate_quantitative_measurements_of_MGMT_status_in_human_cells/7777952）。