一项使用下一代测序在晚期癌症中的初步研究：可行性与挑战

数据集获取（Figshare）

• 源站标题：A Pilot Study Using Next-Generation Sequencing in Advanced Cancers: Feasibility and Challenges
• DOI：10.1371/journal.pone.0076438
• 发布日期：2013-10-30
• 文件数：12 个
• 文件总体积：26.91 MB
• 源站页面：https://plos.figshare.com/articles/dataset/_A_Pilot_Study_Using_Next_Generation_Sequencing_in_Advanced_Cancers_Feasibility_and_Challenges_/839567

命令行下载

curl -L -o "File S1.DOCX" "https://ndownloader.figshare.com/files/6639645"
curl -L -o "Figure S1.TIFF" "https://ndownloader.figshare.com/files/6639648"
curl -L -o "Figure S2.TIFF" "https://ndownloader.figshare.com/files/6639651"
curl -L -o "Figure S3.TIFF" "https://ndownloader.figshare.com/files/6639654"
curl -L -o "Figure S4.TIFF" "https://ndownloader.figshare.com/files/6639657"
curl -L -o "Figure S5.TIFF" "https://ndownloader.figshare.com/files/6639660"
curl -L -o "Figure S6.TIFF" "https://ndownloader.figshare.com/files/6639663"
curl -L -o "Figure S7.TIFF" "https://ndownloader.figshare.com/files/6639666"

文件清单

• File S1.DOCX（30.49 KB）
• Figure S1.TIFF（1.95 MB）
• Figure S2.TIFF（4.67 MB）
• Figure S3.TIFF（5.92 MB）
• Figure S4.TIFF（3.94 MB）
• Figure S5.TIFF（2.57 MB）
• Figure S6.TIFF（1.35 MB）
• Figure S7.TIFF（4.15 MB）
• Figure S8.TIFF（2.26 MB）
• Table S1.XLSX（17.73 KB）
• Table S2.XLSX（28.21 KB）
• Table S3.XLSX（8.68 KB）

文件总体积 26.91 MB（源站 API 实测）。

数据说明

Purpose
New anticancer agents that target a single cell surface receptor, up-regulated or amplified gene product, or mutated gene, have met with some success in treating advanced cancers. However, patients’’ tumors still eventually progress on these therapies. If it were possible to identify a larger number of targetable vulnerabilities in an individual’‘s tumor, multiple targets could be exploited with the use of specific therapeutic agents, thus possibly giving the patient viable therapeutic alternatives.
Experimental Design
In this exploratory study, we used next-generation sequencing technologies (NGS) including whole genome sequencing (WGS), and where feasible, whole transcriptome sequencing (WTS) to identify genomic events and associated expression changes in advanced cancer patients.
Results
WGS on paired tumor and normal samples from nine advanced cancer patients and WTS on six of these patients’’ tumors was completed. One patient’'s treatment was based on targets and pathways identified by NGS and the patient had a short-lived PET/CT response with a significant reduction in his tumor-related pain. To design treatment plans based on information garnered from NGS, several challenges were encountered: NGS reporting delays, communication of results to out-of-state participants and their treating oncologists, and chain of custody handling for fresh biopsy samples for Clinical Laboratory Improvement Amendments (CLIA) target validation.
Conclusion
While the initial effort was a slower process than anticipated due to a variety of issues, we demonstrate the feasibility of using NGS in advanced cancer patients so that treatments for patients with progressing tumors may be improved.

许可

CC BY 4.0

引用

学术使用请引用 DOI 10.1371/journal.pone.0076438（source: https://plos.figshare.com/articles/dataset/_A_Pilot_Study_Using_Next_Generation_Sequencing_in_Advanced_Cancers_Feasibility_and_Challenges_/839567）。