变异克雅氏病异常朊蛋白检测数据集（钢表面PMCA法）

数据集获取（Figshare）

• 源站标题：Rapid and Highly Sensitive Detection of Variant Creutzfeldt - Jakob Disease Abnormal Prion Protein on Steel Surfaces by Protein Misfolding Cyclic Amplification: Application to Prion Decontamination Studies
• DOI：10.1371/journal.pone.0146833
• 发布日期：2016-01-28
• 文件数：2 个
• 文件总体积：345.21 KB
• 源站页面：https://plos.figshare.com/articles/dataset/_Rapid_and_Highly_Sensitive_Detection_of_Variant_Creutzfeldt_Jakob_Disease_Abnormal_Prion_Protein_on_Steel_Surfaces_by_Protein_Misfolding_Cyclic_Amplification_Application_to_Prion_Decontamination_Studies_/1640854

命令行下载

curl -L -o "S1_Fig.docx" "https://ndownloader.figshare.com/files/2632220"
curl -L -o "S1_Table.docx" "https://ndownloader.figshare.com/files/2632221"

文件清单

• S1_Fig.docx（326.30 KB）
• S1_Table.docx（18.91 KB）

文件总体积 345.21 KB（源站 API 实测）。

数据加载示例（图像类）

from PIL import Image
import glob, os

files = (glob.glob(os.path.join(path, "**", "*.png"), recursive=True)
       + glob.glob(os.path.join(path, "**", "*.jpg"), recursive=True)
       + glob.glob(os.path.join(path, "**", "*.tif"), recursive=True))
print("图像文件数:", len(files))
img = Image.open(files[0]); print("尺寸/模式:", img.size, img.mode)

# torchvision Dataset 方式:
# from torchvision import datasets
# ds = datasets.ImageFolder(path)  # 要求 子目录=类别

目录组织与标注格式以源站说明和下载后实际文件为准。

数据说明

The prevalence of variant Creutzfeldt-Jakob disease (vCJD) in the population remains uncertain, although it has been estimated that 1 in 2000 people in the United Kingdom are positive for abnormal prion protein (PrPTSE) by a recent survey of archived appendix tissues. The prominent lymphotropism of vCJD prions raises the possibility that some surgical procedures may be at risk of iatrogenic vCJD transmission in healthcare facilities. It is therefore vital that decontamination procedures applied to medical devices before their reprocessing are thoroughly validated. A current limitation is the lack of a rapid model permissive to human prions. Here, we developed a prion detection assay based on protein misfolding cyclic amplification (PMCA) technology combined with stainless-steel wire surfaces as carriers of prions (Surf-PMCA). This assay allowed the specific detection of minute quantities (10−8 brain dilution) of either human vCJD or ovine scrapie PrPTSE adsorbed onto a single steel wire, within a two week timeframe. Using Surf-PMCA we evaluated the performance of several reference and commercially available prion-specific decontamination procedures. Surprisingly, we found the efficiency of several marketed reagents to remove human vCJD PrPTSE was lower than expected. Overall, our results demonstrate that Surf-PMCA can be used as a rapid and ultrasensitive assay for the detection of human vCJD PrPTSE adsorbed onto a metallic surface, therefore facilitating the development and validation of decontamination procedures against human prions.

许可

CC BY 4.0

引用

学术使用请引用 DOI 10.1371/journal.pone.0146833（source: https://plos.figshare.com/articles/dataset/_Rapid_and_Highly_Sensitive_Detection_of_Variant_Creutzfeldt_Jakob_Disease_Abnormal_Prion_Protein_on_Steel_Surfaces_by_Protein_Misfolding_Cyclic_Amplification_Application_to_Prion_Decontamination_Studies_/1640854）。