被招募与驻留肿瘤相关巨噬细胞对肿瘤控制的双重作用

数据集获取

源站：https://ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE313081

标准获取流程：

1. 访问源站链接，注册/登录账号；
2. 进入数据集详情页，按页面指引申请权限或直接下载；
3. 部分平台提供 SDK，例如：
   • OpenDataLab: pip install openxlab 后 openxlab.dataset.get(...)
   • ModelScope: pip install modelscope 后 modelscope download
   • 天池: 登录后页面提供直链下载
     以源站「数据下载/使用文档」页面为准。

数据说明

Macrophages are among the most abundant immune cells in solid tumors, yet how their diverse origins and spatial organization shape anti-tumor immunity remains unclear. Here, we uncover a fundamental division of labor between tissue-resident interstitial macrophage (IM) subsets and recruited macrophages (recMacs) in lung cancer. Using single-cell and spatial transcriptomics, we identify chemokine-defined IM subsets with opposing functions: Cxcl13-, Cxcl9-, and Cxcl10-producing CD206hi IMs positioned along bronchovascular regions drive tertiary lymphoid structure formation, lymphocyte recruitment, and tumor control, whereas Ccl2-expressing IMs localize within tumor regions and recruit pro-tumorigenic recMacs. We further show that recMac-derived moDCs function as immunosuppressive antigen-presenting cells in draining lymph nodes. During neoantigen vaccination, CCR5 inhibition with Maraviroc selectively blocks antigen-bearing monocyte but not dendritic cell migration, thereby enhancing dendritic cell-mediated tumor immunity. These findings reveal how macrophage ontogeny and spatial compartmentalization govern tumor immunity and point to therapeutic strategies that preserve protective IM functions while disrupting recMac-driven immunosuppression.

数据加载示例（图像类）

from PIL import Image
import glob, os

files = (glob.glob(os.path.join(path, "**", "*.png"), recursive=True)
       + glob.glob(os.path.join(path, "**", "*.jpg"), recursive=True)
       + glob.glob(os.path.join(path, "**", "*.tif"), recursive=True))
print("图像文件数:", len(files))
img = Image.open(files[0]); print("尺寸/模式:", img.size, img.mode)

# torchvision Dataset 方式:
# from torchvision import datasets
# ds = datasets.ImageFolder(path)  # 要求 子目录=类别

目录组织与标注格式以源站说明和下载后实际文件为准。