肺腺癌起源细胞改变髓系介导的免疫抑制

数据集获取

源站：https://ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE267731

标准获取流程：

1. 访问源站链接，注册/登录账号；
2. 进入数据集详情页，按页面指引申请权限或直接下载；
3. 部分平台提供 SDK，例如：
   • OpenDataLab: pip install openxlab 后 openxlab.dataset.get(...)
   • ModelScope: pip install modelscope 后 modelscope download
   • 天池: 登录后页面提供直链下载
     以源站「数据下载/使用文档」页面为准。

数据说明

Lung adenocarcinoma (LUAD) is the most prevalent subtype of lung cancer with extensive biological heterogeneity, such as varying cells of origin from alveolar epithelial cells (AECs) in the distal lung. The predominant types of AECs include surfactant-producing alveolar epithelial type II (AT2) cells and large, delicate alveolar epithelial type I (AT1) cells, which are responsible for facilitating gas exchange. Although AT1 cells have been thought to be terminally differentiated and consequently unable to proliferate, we, and others, have previously reported that Gramd2+ AT1 cells can give rise to a histologically-defined LUAD1,2 on transgenic mouse model. Immunotherapy has shown a major impact on treatment of solid tumors over the past 10 years, particularly on LUAD, where it significantly improves patients’ survival rates3. However, because of the largely unknown immunosuppressive states of LUAD, there is considerable variability in patient response to therapy across LUAD patients, making immunotherapeutic treatment challenging. To determine whether cells of origin for LUAD reprogram differently tumor immune microenvironment (TIME) landscape, we employed single nucleus RNA sequencing (snRNA-Seq) on mouse KRASG12D-overexpressing Gramd2+ AT1 cell and Sftpc+ AT2 cell-derived LUADs to comprehensively characterize transcriptomic, molecular, and cellular aberrations within the TIME. We demonstrate that myeloid cells within the AT1-LUAD TIME manifest a disordered cell composition and disrupted transcriptome: increased cell number of inflammatory monocytes and macrophages but decreased cell number of myeloid-derived suppressor cells (MDSCs) were observed in AT1-LUAD TIME compared with its AT2 counterpart. In addition, a series of bioinformatic analyses, gene set enrichment analysis (GSEA), ingenuity pathway analysis (IPA) and cell-cell communication (CCC) analysis, along with immunostaining confirmed LUAD TIME derived from different cells of origin exhibits altered suppre

数据加载示例（图像类）

from PIL import Image
import glob, os

files = (glob.glob(os.path.join(path, "**", "*.png"), recursive=True)
       + glob.glob(os.path.join(path, "**", "*.jpg"), recursive=True)
       + glob.glob(os.path.join(path, "**", "*.tif"), recursive=True))
print("图像文件数:", len(files))
img = Image.open(files[0]); print("尺寸/模式:", img.size, img.mode)

# torchvision Dataset 方式:
# from torchvision import datasets
# ds = datasets.ImageFolder(path)  # 要求 子目录=类别

目录组织与标注格式以源站说明和下载后实际文件为准。