下一代MUT-MAP：高灵敏度高通量微流体芯片突变分析面板

数据集获取（Figshare）

• 源站标题：Next Generation MUT-MAP, a High-Sensitivity High-Throughput Microfluidics Chip-Based Mutation Analysis Panel
• DOI：10.1371/journal.pone.0090761
• 发布日期：2014-03-21
• 文件数：3 个
• 文件总体积：792.00 KB
• 源站页面：https://plos.figshare.com/articles/dataset/_Next_Generation_MUT_MAP_a_High_Sensitivity_High_Throughput_Microfluidics_Chip_Based_Mutation_Analysis_Panel_/970061

命令行下载

curl -L -o "Figure_S1.tif" "https://ndownloader.figshare.com/files/1430225"
curl -L -o "Table_S1.xlsx" "https://ndownloader.figshare.com/files/1430226"
curl -L -o "Table_S2.xlsx" "https://ndownloader.figshare.com/files/1430227"

文件清单

• Figure_S1.tif（732.23 KB）
• Table_S1.xlsx（42.75 KB）
• Table_S2.xlsx（17.03 KB）

文件总体积 792.00 KB（源站 API 实测）。

数据加载示例（图像类）

from PIL import Image
import glob, os

files = (glob.glob(os.path.join(path, "**", "*.png"), recursive=True)
       + glob.glob(os.path.join(path, "**", "*.jpg"), recursive=True)
       + glob.glob(os.path.join(path, "**", "*.tif"), recursive=True))
print("图像文件数:", len(files))
img = Image.open(files[0]); print("尺寸/模式:", img.size, img.mode)

# torchvision Dataset 方式:
# from torchvision import datasets
# ds = datasets.ImageFolder(path)  # 要求 子目录=类别

目录组织与标注格式以源站说明和下载后实际文件为准。

数据说明

Molecular profiling of tumor tissue to detect alterations, such as oncogenic mutations, plays a vital role in determining treatment options in oncology. Hence, there is an increasing need for a robust and high-throughput technology to detect oncogenic hotspot mutations. Although commercial assays are available to detect genetic alterations in single genes, only a limited amount of tissue is often available from patients, requiring multiplexing to allow for simultaneous detection of mutations in many genes using low DNA input. Even though next-generation sequencing (NGS) platforms provide powerful tools for this purpose, they face challenges such as high cost, large DNA input requirement, complex data analysis, and long turnaround times, limiting their use in clinical settings. We report the development of the next generation mutation multi-analyte panel (MUT-MAP), a high-throughput microfluidic, panel for detecting 120 somatic mutations across eleven genes of therapeutic interest (AKT1, BRAF, EGFR, FGFR3, FLT3, HRAS, KIT, KRAS, MET, NRAS, and PIK3CA) using allele-specific PCR (AS-PCR) and Taqman technology. This mutation panel requires as little as 2 ng of high quality DNA from fresh frozen or 100 ng of DNA from formalin-fixed paraffin-embedded (FFPE) tissues. Mutation calls, including an automated data analysis process, have been implemented to run 88 samples per day. Validation of this platform using plasmids showed robust signal and low cross-reactivity in all of the newly added assays and mutation calls in cell line samples were found to be consistent with the Catalogue of Somatic Mutations in Cancer (COSMIC) database allowing for direct comparison of our platform to Sanger sequencing. High correlation with NGS when compared to the SuraSeq500 panel run on the Ion Torrent platform in a FFPE dilution experiment showed assay sensitivity down to 0.45%. This multiplexed mutation panel is a valuable tool for high-throughput biomarker discovery in personalized medicine and cancer drug development.

许可

CC BY 4.0

引用

学术使用请引用 DOI 10.1371/journal.pone.0090761（source: https://plos.figshare.com/articles/dataset/_Next_Generation_MUT_MAP_a_High_Sensitivity_High_Throughput_Microfluidics_Chip_Based_Mutation_Analysis_Panel_/970061）。