电磁导航支气管镜标本的激酶组学分析：个性化医疗新方法

数据集获取（Figshare）

• 源站标题：Kinomic Profiling of Electromagnetic Navigational Bronchoscopy Specimens: A New Approach for Personalized Medicine
• DOI：10.1371/journal.pone.0116388
• 发布日期：2014-12-30
• 文件数：2 个
• 文件总体积：6.64 MB
• 源站页面：https://plos.figshare.com/articles/dataset/_Kinomic_Profiling_of_Electromagnetic_Navigational_Bronchoscopy_Specimens_A_New_Approach_for_Personalized_Medicine_/1282160

命令行下载

curl -L -o "S1_Fig.tif" "https://ndownloader.figshare.com/files/1858063"
curl -L -o "S2_Fig.tif" "https://ndownloader.figshare.com/files/1858064"

文件清单

• S1_Fig.tif（3.12 MB）
• S2_Fig.tif（3.52 MB）

文件总体积 6.64 MB（源站 API 实测）。

数据加载示例（图像类）

from PIL import Image
import glob, os

files = (glob.glob(os.path.join(path, "**", "*.png"), recursive=True)
       + glob.glob(os.path.join(path, "**", "*.jpg"), recursive=True)
       + glob.glob(os.path.join(path, "**", "*.tif"), recursive=True))
print("图像文件数:", len(files))
img = Image.open(files[0]); print("尺寸/模式:", img.size, img.mode)

# torchvision Dataset 方式:
# from torchvision import datasets
# ds = datasets.ImageFolder(path)  # 要求 子目录=类别

目录组织与标注格式以源站说明和下载后实际文件为准。

数据说明

Purpose
Researchers are currently seeking relevant lung cancer biomarkers in order to make informed decisions regarding therapeutic selection for patients in so-called “precision medicine.” However, there are challenges to obtaining adequate lung cancer tissue for molecular analyses. Furthermore, current molecular testing of tumors at the genomic or transcriptomic level are very indirect measures of biological response to a drug, particularly for small molecule inhibitors that target kinases. Kinase activity profiling is therefore theorized to be more reflective of invivo biology than many current molecular analysis techniques. As a result, this study seeks to prove the feasibility of combining a novel minimally invasive biopsy technique that expands the number of lesions amenable for biopsy with subsequent exvivo kinase activity analysis.
Methods
Eight patients with lung lesions of varying location and size were biopsied using the novel electromagnetic navigational bronchoscopy (ENB) technique. Basal kinase activity (kinomic) profiles and exvivo interrogation of samples in combination with tyrosine kinase inhibitors erlotinib, crizotinib, and lapatinib were performed by PamStation 12 microarray analysis.
Results
Kinomic profiling qualitatively identified patient specific kinase activity profiles as well as patient and drug specific changes in kinase activity profiles following exposure to inhibitor. Thus, the study has verified the feasibility of ENB as a method for obtaining tissue in adequate quantities for kinomic analysis and has demonstrated the possible use of this tissue acquisition and analysis technique as a method for future study of lung cancer biomarkers.
Conclusions
We demonstrate the feasibility of using ENB-derived biopsies to perform kinase activity assessment in lung cancer patients.

许可

CC BY 4.0

引用

学术使用请引用 DOI 10.1371/journal.pone.0116388（source: https://plos.figshare.com/articles/dataset/_Kinomic_Profiling_of_Electromagnetic_Navigational_Bronchoscopy_Specimens_A_New_Approach_for_Personalized_Medicine_/1282160）。