类风湿关节炎患者血清学状态与两种循环蛋白生物标志物关联表（Table_4）

数据集获取（Figshare）

• 源站标题：Table_4_Association of the serological status of rheumatoid arthritis patients with two circulating protein biomarkers: A useful tool for precision medicine strategies.xlsx
• DOI：10.3389/fmed.2022.963540.s005
• 发布日期：2022-10-28
• 文件数：1 个
• 文件总体积：127.33 KB
• 源站页面：https://frontiersin.figshare.com/articles/dataset/Table_4_Association_of_the_serological_status_of_rheumatoid_arthritis_patients_with_two_circulating_protein_biomarkers_A_useful_tool_for_precision_medicine_strategies_xlsx/21426558

命令行下载

curl -L -o "Table_4_Association of the serological status of rheumatoid arthritis patients with two circulating protein biomarkers: A useful tool for precision me.xlsx" "https://ndownloader.figshare.com/files/38018352"

文件清单

• Table_4_Association of the serological status of rheumatoid arthritis patients with two circulating protein biomarkers: A useful tool for precision me.xlsx（127.33 KB）

文件总体积 127.33 KB（源站 API 实测）。

数据说明

Rheumatoid arthritis (RA) is an autoimmune disease characterized by chronic inflammation of the joints and presence of systemic autoantibodies, with a great clinical and molecular heterogeneity. Rheumatoid Factor (RF) and anti-citrullinated protein antibodies (ACPA) are routinely used for the diagnosis of RA. However, additional serological markers are needed to improve the clinical management of this disease, allowing for better patient stratification and the desirable application of precision medicine strategies. In the present study, we investigated those systemic molecular changes that are associated with the RF and ACPA status of RA patients. To achieve this objective, we followed a proteomic biomarker pipeline from the discovery phase to validation. First, we performed an iTRAQ-based quantitative proteomic experiment on serum samples from the RA cohort of the Hospital of Santiago de Compostela (CHUS). In this discovery phase, serum samples from the CHUS cohort were pooled according to their RF/ACPA status. Shotgun analysis revealed that, in comparison with the double negative group (RF–/ACPA–), the abundance of 12 proteins was altered in the RF+/ACPA+ pool, 16 in the RF+/ACPA– pool and 10 in the RF-/ACPA+ pool. Vitamin D binding protein and haptoglobin were the unique proteins increased in all the comparisons. For the verification phase, 80 samples from the same cohort were analyzed individually. To this end, we developed a Multiple Reaction Monitoring (MRM) method that was employed in a comprehensive targeted analysis with the aim of verifying the results obtained in the discovery phase. Thirty-one peptides belonging to 12 proteins associated with RF and/or ACPA status were quantified by MRM. In a final validation phase, the serum levels of alpha-1-acid glycoprotein 1 (A1AG1), haptoglobin (HPT) and retinol-binding protein 4 (RET4) were measured by immunoassays in the RA cohort of the Hospital of A Coruña (HUAC). The increase of two of these putative biomarkers in the double seropositive group was validated in 260 patients from this cohort (p = 0.009 A1AG1; p = 0.003 HPT). The increased level of A1AG1 showed association with RF rather than ACPA (p = 0.023)

许可

CC BY 4.0

引用

学术使用请引用 DOI 10.3389/fmed.2022.963540.s005（source: https://frontiersin.figshare.com/articles/dataset/Table_4_Association_of_the_serological_status_of_rheumatoid_arthritis_patients_with_two_circulating_protein_biomarkers_A_useful_tool_for_precision_medicine_strategies_xlsx/21426558）。