利用淬灭模式罗丹明123检测寡霉素对胰岛素瘤细胞线粒体膜电位影响的数据集

资源简介

利用淬灭模式罗丹明123检测寡霉素对胰岛素瘤细胞线粒体膜电位影响的数据集

【数据集背景】

本数据集源站标题为《Assaying the effect of oligomycin on ΔψM using rhodamine 123 in quench mode in insulinoma cells results in internally inconsistent data.》。源站首次发布：2016-07-12。

【数据内容】

源站原始描述：(A) Whole cell fluorescence, (B) mitochondrial fluorescence obtained by high pass filtering and (C) fluorescence over the nuclei in rhodamine 123-loaded INS-1E cells following the additions of glucose (30 mM), oligomycin (2 μg/ml) and FCCP (1 μM). Data are mean±SE of n = 3 experiments. Note that the oligomycin-evoked drop in quench-mode whole-cell fluorescence (A) originates from the mitochondria (B) and not from the cytosol (approximated by rhodamine 123 fluorescence in the nucleosol) (C). (D)…

【数据结构与技术规格】

文件数 1 个，合计 4.13 MB。Fig 7.TIF：image/tiff，4.13 MB。数据集 DOI：10.1371/journal.pone.0159199.g007。

【主题与分类】

源站学科分类：Biophysics、Biochemistry、Cell Biology、Genetics、Molecular Biology、Physiology。主题标签：Dispersed islet cell cultures、Time Courses、glucose heterogeneously、rhodamine 123.、insulin secretion、Absolute Magnitude、rhodamine 123 assay、biphasic response、tetramethylrhodamine methyl ester、mitochondrial membrane。

【适用方向】

源站学科分类标注为 Biophysics、Biochemistry、Cell Biology、Genetics、Molecular Biology、Physiology。

【获取与许可】

源站页面：https://plos.figshare.com/articles/dataset/Assaying_the_effect_of_oligomycin_on_M_using_rhodamine_123_in_quench_mode_in_insulinoma_cells_results_in_internally_inconsistent_data_/3483593

使用许可：CC BY 4.0（https://creativecommons.org/licenses/by/4.0/）。

规范引用：Gerencser, Akos A.; A. Mookerjee, Shona; Jastroch, Martin; Brand, Martin D. (2016). Assaying the effect of oligomycin on ΔψM using rhodamine 123 in quench mode in insulinoma cells results in internally inconsistent data.. PLOS ONE. Dataset

【使用注意】

· 源站最后修改于 2023-06-01，引用时请注意版本时效。