一种用于识别胰岛素产生β细胞及分类胰岛素瘤细胞系异质性的新型双色报告系统

资源简介

一种用于识别胰岛素产生β细胞及分类胰岛素瘤细胞系异质性的新型双色报告系统

【数据集背景】

本数据集源站标题为《A Novel Dual-Color Reporter for Identifying Insulin-Producing Beta- Cells and Classifying Heterogeneity of Insulinoma Cell Lines》，为 Insulinoma Cell Lines 的组成部分。配套论文《A Novel Dual-Color Reporter for Identifying Insulin-Producing Beta- Cells and Classifying Heterogeneity of Insulinoma Cell Lines》发表于 PLoS ONE（2012），DOI：10.1371/journal.pone.0035521。源站首次发布：2012-04-18。

【数据内容】

源站原始描述：Many research studies use immortalized cell lines as surrogates for primary beta- cells. We describe the production and use of a novel “indirect" dual-fluorescent reporter system that leads to mutually exclusive expression of EGFP in insulin-producing (INS+) beta-cells or mCherry in non-beta-cells. Our system uses the human insulin promoter to initiate a Cre-mediated shift in reporter color within a single transgene construct and is useful for FACS selection of cells from single cultures for fur…

【数据结构与技术规格】

文件数 2 个，合计 2.71 MB。Figure_S1.doc：application/CDFV2，1.66 MB。Figure_S2.doc：application/msword，1.05 MB。数据集 DOI：10.1371/journal.pone.0035521。

【主题与分类】

源站学科分类：Chemistry、Biochemistry、Developmental Biology、Biophysics、Physiology、Biotechnology。主题标签：dual-color、identifying、insulin-producing、beta-、cells、classifying、heterogeneity、insulinoma、lines。

【适用方向】

源站学科分类标注为 Chemistry、Biochemistry、Developmental Biology、Biophysics、Physiology、Biotechnology。

【获取与许可】

源站页面：https://plos.figshare.com/articles/dataset/A_Novel_Dual_Color_Reporter_for_Identifying_Insulin_Producing_Beta_Cells_and_Classifying_Heterogeneity_of_Insulinoma_Cell_Lines/126316

使用许可：CC BY 4.0（https://creativecommons.org/licenses/by/4.0/）。

规范引用：Sook Lee, Nan; G. Rohan, Joyce; Zitting, Madison; Kamath, Sonia; Weitz, Andrew; Sipos, Arnold; et al. (2016). A Novel Dual-Color Reporter for Identifying Insulin-Producing Beta- Cells and Classifying Heterogeneity of Insulinoma Cell Lines. PLOS ONE. Dataset

【使用注意】

· 源站最后修改于 2023-05-31，引用时请注意版本时效。