DataSheet_2_个体化条件重编程系统指导胃癌治疗的应用研究

数据集获取（Figshare）

• 源站标题：DataSheet_2_Application Research of Individualized Conditional Reprogramming System to Guide Treatment of Gastric Cancer.xlsx
• DOI：10.3389/fonc.2021.709511.s002
• 发布日期：2021-07-16
• 文件数：1 个
• 文件总体积：33.17 KB
• 源站页面：https://frontiersin.figshare.com/articles/dataset/DataSheet_2_Application_Research_of_Individualized_Conditional_Reprogramming_System_to_Guide_Treatment_of_Gastric_Cancer_xlsx/14993571

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curl -L -o "DataSheet_2_Application Research of Individualized Conditional Reprogramming System to Guide Treatment of Gastric Cancer.xlsx" "https://ndownloader.figshare.com/files/28860630"

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• DataSheet_2_Application Research of Individualized Conditional Reprogramming System to Guide Treatment of Gastric Cancer.xlsx（33.17 KB）

文件总体积 33.17 KB（源站 API 实测）。

数据说明

BackgroundGastric cancer (GC) is one of the most common causes of malignant tumors in the world. Due to the high heterogeneity of GC and lack of specificity of available chemotherapy regimens, these tumors are prone to resistance, recurrence, and metastasis. Here, we formulated an individualized chemotherapy regimen for GC using a modified individual conditional reprogramming (i-CR) system. We established a primary tumor cell bank of GC cells and completed drug screening in order to realize individualized and accurate GC treatment.
MethodsWe collected specimens from 93 surgical or gastroscopy GC cases and established a primary tumor cell bank using the i-CR system and PDX models. We also completed in vitro culture and drug sensitivity screening of the GC cells using the i-CR system. Whole-exome sequencing (WES) of the i-CR cells was performed using P0 and P5. We then chose targeted chemotherapy drugs based on the i-CR system results.
ResultsOf the 72 cases that were collected from surgical specimens, 26 cases were successfully cultured with i-CR system, and of the 21 cases collected from gastroscopy specimens, seven were successfully cultured. Among these, 20 cases of the PDX model were established. SRC ± G3 had the highest culture success rate. The i-CR cells of P0 and P5 appeared to be highly conserved. According to drug sensitivity screening, we examined the predictive value of responses of GC patients to chemotherapeutic agents, especially in neoadjuvant patients.
ConclusionThe i-CR system does not only represent the growth characteristics of tumors in vivo, but also provides support for clinical drug use. Drug susceptibility results were relatively consistent with clinical efficacy.

许可

CC BY 4.0

引用

学术使用请引用 DOI 10.3389/fonc.2021.709511.s002（source: https://frontiersin.figshare.com/articles/dataset/DataSheet_2_Application_Research_of_Individualized_Conditional_Reprogramming_System_to_Guide_Treatment_of_Gastric_Cancer_xlsx/14993571）。