非小细胞肺癌靶向NGS panel验证数据集

数据集获取（Figshare）

• 源站标题：Table5_Development, testing and validation of a targeted NGS-panel for the detection of actionable mutations in lung cancer (NSCLC) using anchored multiplex PCR technology in a multicentric setting.XLSX
• DOI：10.3389/pore.2024.1611590.s005
• 发布日期：2024-03-28
• 文件数：1 个
• 文件总体积：13.75 KB
• 源站页面：https://frontiersin.figshare.com/articles/dataset/Table5_Development_testing_and_validation_of_a_targeted_NGS-panel_for_the_detection_of_actionable_mutations_in_lung_cancer_NSCLC_using_anchored_multiplex_PCR_technology_in_a_multicentric_setting_XLSX/25494394

命令行下载

curl -L -o "Table5_Development, testing and validation of a targeted NGS-panel for the detection of actionable mutations in lung cancer (NSCLC) using anchored mul.XLSX" "https://ndownloader.figshare.com/files/45325339"

文件清单

• Table5_Development, testing and validation of a targeted NGS-panel for the detection of actionable mutations in lung cancer (NSCLC) using anchored mul.XLSX（13.75 KB）

文件总体积 13.75 KB（源站 API 实测）。

数据加载示例（图像类）

from PIL import Image
import glob, os

files = (glob.glob(os.path.join(path, "**", "*.png"), recursive=True)
       + glob.glob(os.path.join(path, "**", "*.jpg"), recursive=True)
       + glob.glob(os.path.join(path, "**", "*.tif"), recursive=True))
print("图像文件数:", len(files))
img = Image.open(files[0]); print("尺寸/模式:", img.size, img.mode)

# torchvision Dataset 方式:
# from torchvision import datasets
# ds = datasets.ImageFolder(path)  # 要求 子目录=类别

目录组织与标注格式以源站说明和下载后实际文件为准。

数据说明

Lung cancer is a paradigm for a genetically driven tumor. A variety of drugs were developed targeting specific biomarkers requiring testing for tumor genetic alterations in relevant biomarkers. Different next-generation sequencing technologies are available for library generation: 1) anchored multiplex-, 2) amplicon based- and 3) hybrid capture-based-PCR. Anchored multiplex PCR-based sequencing was investigated for routine molecular testing within the national Network Genomic Medicine Lung Cancer (nNGM). Four centers applied the anchored multiplex ArcherDX-Variantplex nNGMv2 panel to re-analyze samples pre-tested during routine diagnostics. Data analyses were performed by each center and compiled centrally according to study design. Pre-defined standards were utilized, and panel sensitivity was determined by dilution experiments. nNGMv2 panel sequencing was successful in 98.9% of the samples (N = 90). With default filter settings, all but two potential MET exon 14 skipping variants were identified at similar allele frequencies. Both MET variants were found with an adapted calling filter. Three additional variants (KEAP1, STK11, TP53) were called that were not identified in pre-testing analyses. Only total DNA amount but not a qPCR-based DNA quality score correlated with average coverage. Analysis was successful with a DNA input as low as 6.25 ng. Anchored multiplex PCR-based sequencing (nNGMv2) and a sophisticated user-friendly Archer-Analysis pipeline is a robust and specific technology to detect tumor genetic mutations for precision medicine of lung cancer patients.

许可

CC BY 4.0

引用

学术使用请引用 DOI 10.3389/pore.2024.1611590.s005（source: https://frontiersin.figshare.com/articles/dataset/Table5_Development_testing_and_validation_of_a_targeted_NGS-panel_for_the_detection_of_actionable_mutations_in_lung_cancer_NSCLC_using_anchored_multiplex_PCR_technology_in_a_multicentric_setting_XLSX/25494394）。