便携式设备用于免实验室通用核酸提取的开发与验证

数据集获取（Figshare）

• 源站标题：Development and validation of a portable device for lab-free versatile nucleic acid extraction
• DOI：10.6084/m9.figshare.27940821.v1
• 发布日期：2024-12-02
• 文件数：2 个
• 文件总体积：23.70 MB
• 源站页面：https://tandf.figshare.com/articles/dataset/Development_and_validation_of_a_portable_device_for_lab-free_versatile_nucleic_acid_extraction/27940821

命令行下载

curl -L -o "Supplementary_Information_w_Highlights.docx" "https://ndownloader.figshare.com/files/50916666"
curl -L -o "Supplementary_Video_V1_480p.mp4" "https://ndownloader.figshare.com/files/50916669"

文件清单

• Supplementary_Information_w_Highlights.docx（15.10 MB）
• Supplementary_Video_V1_480p.mp4（8.61 MB）

文件总体积 23.70 MB（源站 API 实测）。

数据加载示例（图像类）

from PIL import Image
import glob, os

files = (glob.glob(os.path.join(path, "**", "*.png"), recursive=True)
       + glob.glob(os.path.join(path, "**", "*.jpg"), recursive=True)
       + glob.glob(os.path.join(path, "**", "*.tif"), recursive=True))
print("图像文件数:", len(files))
img = Image.open(files[0]); print("尺寸/模式:", img.size, img.mode)

# torchvision Dataset 方式:
# from torchvision import datasets
# ds = datasets.ImageFolder(path)  # 要求 子目录=类别

目录组织与标注格式以源站说明和下载后实际文件为准。

数据说明

Nucleic acid testing (NAT) has revolutionized diagnostics by providing precise, rapid, and scalable detection methods for diverse biological samples. These recent advancements satisfy the increasing demand for on-site diagnostics, yet sample preparation remains a significant bottleneck for achieving highly sensitive diagnostic assays. There is an unmet need for compatible, efficient, and lab-free sample preparation for point-of-care NAT. To address this, we developed a portable, lab-free, and battery-powered device for extracting nucleic acids. We explored using low centrifugal forces with existing commercial chemistry, demonstrating excellent performance. We designed and tested a battery-powered device to enable lab-free extractions, and verified reagents stored out to 6 months, suggesting exceptional deployment capabilities. We evaluated our device, comparing our results against those from a benchtop centrifuge across three types of samples: HIV RNA in buffer, HIV RNA in plasma, and SARS-CoV-2 RNA in saliva. The portable device demonstrated excellent agreement with the benchtop centrifuge, indicating high reliability. By providing an effective on-site sample preparation solution, the widespread adoption of low centrifugal extractions will improve the sensitivity and reliability of NAT and will positively impact other point-of-care technologies such as next generation sequencing (NGS), biomarker detection, and environmental monitoring.
This method utilizes a low-power, portable centrifuge to significantly improve the deployment of nucleic acid extractions. As a result, this method offers comparable extraction performance to benchtop devices while offering superior portability and ease of use. Minimizing centrifugal force allows for reliable nucleic acid extraction from a low-power device. Our approach is simple and uses low-cost electronics, presenting high potential for clinical preparation of RNA in field settings.

Sample preparation continues to be a major bottleneck for sensitive diagnostic assays.
We developed a portable, lab-free, and battery-powered device for extracting nucleic acids.
We demonstrated that a commercial extraction kit could be processe

许可

CC BY 4.0

引用

学术使用请引用 DOI 10.6084/m9.figshare.27940821.v1（source: https://tandf.figshare.com/articles/dataset/Development_and_validation_of_a_portable_device_for_lab-free_versatile_nucleic_acid_extraction/27940821）。